{"id":37380,"date":"2025-01-27T13:00:41","date_gmt":"2025-01-27T05:00:41","guid":{"rendered":"https:\/\/17aitech.com\/?p=37380"},"modified":"2025-01-27T13:00:41","modified_gmt":"2025-01-27T05:00:41","slug":"%e9%ba%a6%e5%90%89%e5%b0%94%e5%a4%a7%e5%ad%a6ding-lab%e5%9f%ba%e4%ba%8e%e6%b7%b1%e5%ba%a6%e5%ad%a6%e4%b9%a0%e5%bc%80%e5%8f%91%e5%8d%95%e7%bb%86%e8%83%9e%e6%b0%b4%e5%b9%b3%e8%bd%ac%e5%ba%a7%e5%ad%90","status":"publish","type":"post","link":"https:\/\/17aitech.com\/?p=37380","title":{"rendered":"\u9ea6\u5409\u5c14\u5927\u5b66Ding Lab\u57fa\u4e8e\u6df1\u5ea6\u5b66\u4e60\u5f00\u53d1\u5355\u7ec6\u80de\u6c34\u5e73\u8f6c\u5ea7\u5b50\u4f4d\u70b9\u8868\u8fbe\u5b9a\u91cf\u6a21\u578b\uff0c\u767bNature\u5b50\u520a"},"content":{"rendered":"

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\u7f16\u8f91\u4e28ScineceAI<\/p>\n

\u8be5\u8bba\u6587\u4ecb\u7ecd MATES\uff1a\u4e00\u79cd\u57fa\u4e8e\u6df1\u5ea6\u5b66\u4e60<\/mark>\u7684\u5355\u7ec6\u80de\u6c34\u5e73\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u5de5\u5177\u3002MATES \u4f7f\u7528\u57fa\u4e8e\u81ea\u7f16\u7801\u5668\u7684\u6a21\u578b\uff0c\u901a\u8fc7\u5206\u6790\u8f6c\u5ea7\u5b50\u533a\u57df\u5468\u56f4\u72ec\u7279\u6bd4\u5bf9\u8bfb\u6bb5\u7684\u5206\u5e03\uff0c\u6982\u7387\u6027\u5730\u5c06\u591a\u91cd\u6bd4\u5bf9\u8f6c\u5ea7\u5b50\u8bfb\u6bb5\u5206\u914d\u5230\u7279\u5b9a\u4f4d\u70b9\u3002\u901a\u8fc7\u6df1\u5ea6\u795e\u7ecf\u7f51\u7edc<\/mark>\uff0cMATES \u5b66\u4e60\u72ec\u7279\u8bfb\u6bb5\u5206\u5e03\u4e0e\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u6765\u81ea\u7279\u5b9a\u4f4d\u70b9\u7684\u53ef\u80fd\u6027\u4e4b\u95f4\u7684\u5173\u7cfb\u3002<\/p>\n

\u8fd9\u4e00\u521b\u65b0\u65b9\u6cd5\u5728\u591a\u4e2a\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f0\u4e0a\u8fdb\u884c\u4e86\u4e25\u683c\u9a8c\u8bc1\uff0c\u5305\u62ec 10X Genomics\uff08scRNA-seq\u3001scATAC-seq \u548c Multiome\uff09\u3001Smart-seq \u548c\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\uff0810X Visium\uff09\u3002\u8be5\u5de5\u5177\u7684\u591a\u6837\u6027\u548c\u7cbe\u786e\u6027\u5df2\u5e26\u6765\u4e86\u65b0\u7684\u751f\u7269\u5b66\u89c1\u89e3\uff0c\u4e3a\u66f4\u5e7f\u6cdb\u7684\u5e94\u7528\u548c\u5b9e\u9a8c\u9a8c\u8bc1\u94fa\u5e73\u4e86\u9053\u8def\u3002<\/p>\n

\u8be5\u7814\u7a76\u4ee5\u300cMATES: a deep learning-based model for locus-specific quantification of transposable elements in single cell<\/em>\u300d\u4e3a\u9898\uff0c\u4e8e 2024 \u5e74 10 \u6708 11 \u65e5\u53d1\u5e03\u5728\u300aNature Communications<\/em>\u300b\u3002<\/p>\n

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\u80cc\u666f\u4ecb\u7ecd<\/strong><\/p>\n

\u8f6c\u5ea7\u5b50(Transposon)\uff0c\u53c8\u79f0\u8f6c\u5ea7\u5143\u4ef6\u6216\u8df3\u8dc3\u57fa\u56e0\uff0c\u662f\u54fa\u4e73\u52a8\u7269\u57fa\u56e0\u7ec4\u7684\u91cd\u8981\u7ec4\u6210\u90e8\u5206\uff0c\u5728\u57fa\u56e0\u8c03\u63a7\u3001\u57fa\u56e0\u7ec4\u8fdb\u5316\u548c\u7ec6\u80de\u95f4\u5f02\u8d28\u6027\u4e2d\u53d1\u6325\u7740\u5173\u952e\u4f5c\u7528\u3002\u5c3d\u7ba1\u90e8\u5206\u8f6c\u5ea7\u5b50\u4ecd\u7136\u6d3b\u8dc3\u5e76\u80fd\u591f\u5728\u57fa\u56e0\u7ec4\u4e2d\u8df3\u8dc3\uff0c\u4f46\u5927\u591a\u6570\u8f6c\u5ea7\u5b50\u5df2\u7ecf\u79ef\u7d2f\u4e86\u7a81\u53d8\u548c\u9000\u5316\uff0c\u4f7f\u5176\u5931\u53bb\u4e86\u4e3b\u52a8\u8f6c\u5ea7\u7684\u80fd\u529b\u3002\u56e0\u6b64\uff0c\u8bb8\u591a\u8f6c\u5ea7\u5b50\u88ab\u4fdd\u7559\u5728\u57fa\u56e0\u7ec4\u4e2d\u5e76\u4f5c\u4e3a\u8c03\u63a7\u5143\u4ef6\u53d1\u6325\u4f5c\u7528\u3002\u8fd9\u4e9b\u975e\u7f16\u7801\u529f\u80fd\u5305\u62ec\u8c03\u63a7\u57fa\u56e0\u8868\u8fbe\u4ee5\u53ca\u5f62\u6210\u957f\u94fe\u975e\u7f16\u7801 RNA\uff08lncRNA\uff09\uff0c\u8fd9\u4e9b RNA \u53c2\u4e0e\u5173\u952e\u7684\u8c03\u63a7\u7f51\u7edc\uff0c\u5f71\u54cd\u57fa\u56e0\u8868\u8fbe\u548c\u7ec6\u80de\u529f\u80fd\u3002\u5c3d\u7ba1\u8f6c\u5ea7\u5b50\u626e\u6f14\u7740\u8fd9\u4e9b\u91cd\u8981\u89d2\u8272\uff0c\u4f46\u7531\u4e8e\u5176\u91cd\u590d\u5e8f\u5217\u548c\u9ad8\u62f7\u8d1d\u6570\u5bfc\u81f4\u7684\u591a\u91cd\u6bd4\u5bf9\u6d4b\u5e8f\u8bfb\u6bb5\uff08reads\uff09\u5b9a\u91cf\u7684\u6311\u6218\uff0c\u6211\u4eec\u5728\u5355\u7ec6\u80de\u6c34\u5e73\u4e0a\u5bf9\u7279\u5b9a\u4f4d\u70b9\u8f6c\u5ea7\u5b50\u7684\u7406\u89e3\u4ecd\u7136\u6709\u9650\u3002<\/p>\n

\u8f6c\u5ea7\u5b50\u9ad8\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u7684\u6311\u6218<\/strong><\/p>\n

\u4ece\u8f6c\u5ea7\u5b50\u7684\u9ad8\u5bf9\u6bd4\u8bfb\u6bb5\u4e2d\u51c6\u786e\u91cf\u5316<\/mark>\u8868\u8fbe\u91cf\u662f\u8fd9\u4e2a\u9886\u57df\u7684\u4e00\u9879\u91cd\u5927\u6311\u6218\u3002\u8bb8\u591a\u73b0\u6709\u7684\u5355\u7ec6\u80de\u8f6c\u5ea7\u5b50\u91cf\u5316<\/mark>\u5de5\u5177\u8fc7\u5ea6\u4f9d\u8d56\u6bd4\u5bf9\u7b97\u6cd5\u6765\u5904\u7406\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u3002\u7136\u800c\u7531\u4e8e\u8f6c\u5ea7\u5b50\u7684\u91cd\u590d\u6027\uff0c\u5229\u7528\u5bf9\u6bd4\u7b97\u6cd5\u91cf\u5316<\/mark>\u8f6c\u5ea7\u5b50\u8868\u8fbe\u91cf\u5b58\u5728\u5c40\u9650\u6027\uff0c\u5373\u4ed6\u4eec\u5ffd\u7565\u4e86\u8f6c\u5ea7\u5b50\u5468\u56f4\u7684\u57fa\u56e0\u7ec4\u4e0a\u4e0b\u6587\u4fe1\u606f\u3002<\/p>\n

\u7279\u5b9a\u4f4d\u70b9\u8f6c\u5ea7\u5b50\u91cf\u5316<\/mark>\u7684\u6311\u6218<\/strong><\/p>\n

\u73b0\u6709\u7684\u65b9\u6cd5\u672a\u80fd\u5b9e\u73b0\u7cbe\u786e\u7684\u7279\u5b9a\u4f4d\u70b9\u8f6c\u5ea7\u5b50\u5b9a\u91cf\uff0c\u4ed6\u4eec\u6709\u4e9b\u53ea\u91cf\u5316<\/mark>\u8f6c\u5ea7\u5b50\u4e9a\u5bb6\u65cf\u7684\u8868\u8fbe\u91cf\uff0c\u6709\u7684\u4ec5\u4ec5\u5c06\u8bfb\u6bb5\u5206\u914d\u5230\u5bf9\u6bd4\u7b97\u6cd5\u63d0\u4f9b\u7684\u201c\u6700\u4f73\u201d \u4f4d\u7f6e\uff0c\u56e0\u6b64\u5728\u5904\u7406\u8f6c\u5ea7\u5b50\u533a\u57df\u4e2d\u666e\u904d\u5b58\u5728\u7684\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u65b9\u9762\u975e\u5e38\u6709\u9650\u3002\u8fd9\u4e9b\u65b9\u6cd5\u5ffd\u89c6\u6216\u56de\u907f\u4e86\u7531\u8f6c\u5ea7\u5b50\u91cd\u590d\u7279\u6027\u5bfc\u81f4\u7684\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u5206\u914d\u6311\u6218\uff0c\u8fd9\u79cd\u5ffd\u89c6\u53ef\u80fd\u4f4e\u4f30\u4e86\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u4e2d\u5206\u914d\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u7684\u590d\u6742\u6027\u548c\u4e0d\u786e\u5b9a\u6027\u3002<\/p>\n

\u65b9\u6cd5\u603b\u7ed3<\/strong><\/p>\n

\u5229\u7528\u6df1\u5ea6\u5b66\u4e60<\/mark>\u6a21\u578b\u89e3\u51b3\u8f6c\u5ea7\u5b50\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u7684\u6311\u6218<\/strong><\/p>\n

\u4e3a\u4e86\u89e3\u51b3\u8fd9\u4e9b\u6311\u6218\u5e76\u586b\u8865\u7a7a\u767d\uff0c\u6211\u4eec\u63d0\u51fa\u4e86 MATES\uff0c\u8fd9\u662f\u4e00\u79cd\u57fa\u4e8e\u6df1\u5ea6\u795e\u7ecf\u7f51\u7edc<\/mark>\u7684\u65b9\u6cd5\uff0c\u4e13\u4e3a\u8de8\u6a21\u5f0f\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u6570\u636e\u4e2d\u7684\u7279\u5b9a\u4f4d\u70b9\u8f6c\u5ea7\u5b50\u7cbe\u786e\u5b9a\u91cf\u800c\u8bbe\u8ba1\u3002MATES \u5229\u7528\u8f6c\u5ea7\u5b50\u4f4d\u70b9\u5468\u56f4\u72ec\u7279\u6bd4\u5bf9\u8bfb\u6bb5\u7684\u5206\u5e03\u4fe1\u606f\uff0c\u5c06\u591a\u91cd\u6bd4\u5bf9\u8f6c\u5ea7\u5b50\u8bfb\u6bb5\u5206\u914d\u5230\u7279\u5b9a\u4f4d\u70b9\uff0c\u4ece\u800c\u5b9e\u73b0\u7279\u5b9a\u4f4d\u70b9\u8f6c\u5ea7\u5b50\u7684\u5b9a\u91cf\u3002\u901a\u8fc7\u6df1\u5ea6\u795e\u7ecf\u7f51\u7edc<\/mark>\u7684\u5f3a\u5927\u80fd\u529b\uff0cMATES \u6355\u6349\u8f6c\u5ea7\u5b50\u4f4d\u70b9\u5468\u56f4\u72ec\u7279\u6bd4\u5bf9\u8bfb\u6bb5\u5206\u5e03\u4e0e\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u88ab\u5206\u914d\u5230\u8fd9\u4e9b\u4f4d\u70b9\u7684\u6982\u7387\u4e4b\u95f4\u7684\u590d\u6742\u5173\u7cfb\u3002\u8be5\u65b9\u6cd5\u4f7f MATES \u80fd\u591f\u57fa\u4e8e\u8f6c\u5ea7\u5b50\u4f4d\u70b9\u7684\u5c40\u90e8\u4e0a\u4e0b\u6587\uff0c\u4ee5\u6982\u7387\u65b9\u5f0f\u5904\u7406\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u7684\u5206\u914d\u95ee\u9898\u3002<\/p>\n

MATES \u65b9\u6cd5\u5305\u62ec\u591a\u4e2a\u5173\u952e\u6b65\u9aa4\u3002\u9996\u5148\uff0c\u5c06\u539f\u59cb\u8bfb\u6bb5\u6bd4\u5bf9\u5230\u53c2\u8003\u57fa\u56e0\u7ec4\uff0c\u8bc6\u522b\u552f\u4e00\u6bd4\u5bf9\u5230\u67d0\u4e2a\u8f6c\u5ea7\u5b50\u4f4d\u70b9\u7684\u8bfb\u6bb5\uff08\u72ec\u7279\u6bd4\u5bf9\u8bfb\u6bb5\uff09\u4ee5\u53ca\u6bd4\u5bf9\u5230\u591a\u4e2a\u8f6c\u5ea7\u5b50\u4f4d\u70b9\u7684\u8bfb\u6bb5\uff08\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\uff09\u3002\u63a5\u7740\uff0c\u4e3a\u6bcf\u4e2a\u8f6c\u5ea7\u5b50\u4f4d\u70b9\u8ba1\u7b97\u8986\u76d6\u5411\u91cf\uff0c\u8868\u793a\u56f4\u7ed5\u8be5\u4f4d\u70b9\u7684\u72ec\u7279\u6bd4\u5bf9\u8bfb\u6bb5\u5206\u5e03\uff08\u4e0a\u4e0b\u6587\uff09\uff0c\u5e76\u5c06\u6bcf\u4e2a\u8f6c\u5ea7\u5b50\u533a\u57df\u7ec6\u5206\u4e3a\u957f\u5ea6\u4e3a W\uff08\u5982 10 \u4e2a\u78b1\u57fa\u5bf9\uff09\u7684\u8f83\u5c0f\u533a\u95f4\u3002\u6839\u636e\u533a\u95f4\u5185\u72ec\u7279\u6bd4\u5bf9\u8bfb\u6bb5\u548c\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u7684\u6bd4\u4f8b\uff0c\u5c06\u6bcf\u4e2a\u533a\u95f4\u5206\u7c7b\u4e3a\u72ec\u7279\u5360\u4e3b\u5bfc\u533a\u57df\u6216\u591a\u91cd\u5360\u4e3b\u5bfc\u533a\u57df\u3002\u7136\u540e\uff0cMATES \u4f7f\u7528\u81ea\u7f16\u7801\u5668\u6a21\u578b\u5b66\u4e60\u6f5c\u5728\u5d4c\u5165\uff0c\u7528\u4e8e\u8868\u793a\u8f6c\u5ea7\u5b50\u4f4d\u70b9\u7684\u9ad8\u7ef4\u72ec\u7279\u8bfb\u6bb5\u8986\u76d6\u5411\u91cf\uff0c\u5373\u7279\u5b9a\u4f4d\u70b9\u7684\u6bd4\u5bf9\u4e0a\u4e0b\u6587\u3002<\/p>\n

\u6b64\u5916,\u8f6c\u5ea7\u5b50\u5bb6\u65cf\u7684\u72ec\u70ed\u7f16\u7801<\/mark>\u4fe1\u606f\u4e5f\u4f5c\u4e3a\u6a21\u578b\u8f93\u5165\uff0c\u7528\u4e8e\u7ed3\u5408\u6f5c\u5728\u5d4c\u5165\u9884\u6d4b\u7279\u5b9a\u4f4d\u70b9\u7684\u591a\u91cd\u6bd4\u5bf9\u6bd4\u4f8b (\u03b1)\u3002\u6a21\u578b\u7684\u603b\u635f\u5931\u7531\u91cd\u6784<\/mark>\u635f\u5931\u548c\u76f8\u90bb\u533a\u95f4\u8bfb\u6bb5\u8986\u76d6\u8fde\u7eed\u6027\u7684\u635f\u5931\u7ec4\u6210\uff0c\u540e\u8005\u53cd\u6620\u4e86\u591a\u91cd\u5360\u4e3b\u5bfc\u533a\u95f4\u7684\u8986\u76d6\u5e94\u63a5\u8fd1\u5176\u76f8\u90bb\u7684\u72ec\u7279\u5360\u4e3b\u5bfc\u533a\u95f4\u3002\u901a\u8fc7\u8bad\u7ec3\u5b8c\u6210\u7684\u6a21\u578b\uff0c\u6211\u4eec\u53ef\u4ee5\u4ee5\u6982\u7387\u65b9\u5f0f\u7edf\u8ba1\u6bcf\u4e2a\u8f6c\u5ea7\u5b50\u4f4d\u70b9\u7684\u8bfb\u6bb5\u603b\u6570\uff0c\u4ece\u800c\u5b9e\u73b0\u8f6c\u5ea7\u5b50\u5728\u4f4d\u70b9\u6c34\u5e73\u7684\u7cbe\u786e\u5b9a\u91cf\u3002\u8fdb\u4e00\u6b65\u5730\uff0c\u901a\u8fc7\u5c06\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u4e0e\u5355\u7ec6\u80de\u6570\u636e\u4e2d\u7684\u4f20\u7edf\u57fa\u56e0\u5b9a\u91cf\uff08\u5982\u57fa\u56e0\u8868\u8fbe\u6216\u57fa\u56e0\u53ef\u53ca\u6027\uff09\u7ed3\u5408\uff0cMATES \u80fd\u591f\u66f4\u51c6\u786e\u5730\u5bf9\u7ec6\u80de\u8fdb\u884c\u805a\u7c7b<\/mark>\uff0c\u5e76\u8bc6\u522b\u5168\u9762\u7684\u751f\u7269\u6807\u5fd7\u7269\uff08\u57fa\u56e0\u548c\u8f6c\u5ea7\u5b50\uff09\uff0c\u4ee5\u8868\u5f81\u83b7\u5f97\u7684\u7ec6\u80de\u7fa4\u3002\u9664\u4e86\u9ad8\u6548\u5904\u7406\u5355\u7ec6\u80de\u6570\u636e\u7684\u5404\u79cd\u6a21\u5f0f\uff0cMATES \u8fd8\u63d0\u4f9b\u7279\u5b9a\u4f4d\u70b9\u8f6c\u5ea7\u5b50\u7684\u53ef\u89c6\u5316\u529f\u80fd\uff0c\u652f\u6301\u751f\u6210 bigwig \u6587\u4ef6\u548c\u4ea4\u4e92\u5f0f\u57fa\u56e0\u7ec4\u67e5\u770b\u5668\uff08IGV\uff09\u56fe\uff0c\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u76f4\u89c2\u5730\u63a2\u7d22\u548c\u89e3\u91ca\u57fa\u56e0\u7ec4\u4e2d\u8f6c\u5ea7\u5b50\u4f4d\u70b9\u7684\u8bfb\u6bb5\u5206\u914d\u3002<\/p>\n

<\/a><\/p>\n

\u56fe1:MATES\u65b9\u6cd5\u6982\u89c8\u3002<\/p>\n

a\u00a0<\/strong>\u539f\u59cb\u8bfb\u6bb5\u88ab\u6bd4\u5bf9\u5230\u53c2\u8003\u57fa\u56e0\u7ec4\uff0c\u540c\u65f6\u8003\u8651\u8f6c\u5ea7\u5b50\u4f4d\u70b9\u4e0a\u7684\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u3002b<\/strong> \u6784\u5efa\u8f6c\u5ea7\u5b50\u8986\u76d6\u5411\u91cf\uff0c\u5305\u62ec\u72ec\u7279\u6bd4\u5bf9\u8bfb\u6bb5\u8986\u76d6\u5411\u91cf V_u \u548c\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u8986\u76d6\u5411\u91cf V_m\uff0c\u4ee5\u6355\u83b7\u8bfb\u6bb5\u7684\u5206\u5e03\u4fe1\u606f\u3002c<\/strong> \u81ea\u7f16\u7801\u5668\uff08AutoEncoder\uff09\u6a21\u578b\u4ece\u72ec\u7279\u6bd4\u5bf9\u8bfb\u6bb5\u8986\u76d6\u5411\u91cf\u4e2d\u63d0\u53d6\u6f5c\u5728\u5d4c\u5165\u3002\u8fd9\u4e9b\u5d4c\u5165\u4e0e\u8f6c\u5ea7\u5b50\u5bb6\u65cf\u6570\u636e T_i \u7ed3\u5408\uff0c\u7528\u4e8e\u9884\u6d4b\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u4e0e\u6bcf\u4e2a\u8f6c\u5ea7\u5b50\u4f4d\u70b9\u5339\u914d\u7684\u6982\u7387\u03b1\u3002d<\/strong> MATES \u8ba1\u7b97\u7684\u591a\u91cd\u6bd4\u5bf9\u6982\u7387 \u03b1 \u5bf9\u6784\u5efa\u8f6c\u5ea7\u5b50\u8ba1\u6570\u77e9\u9635\u81f3\u5173\u91cd\u8981\u3002\u8be5\u77e9\u9635\u662f\u7ec6\u80de\u5206\u6790\u7684\u5173\u952e\uff0c\u53ef\u5355\u72ec\u4f7f\u7528\uff0c\u4e5f\u53ef\u4e0e\u4f20\u7edf\u57fa\u56e0\u8ba1\u6570\u77e9\u9635\u7ed3\u5408\u4f7f\u7528\u3002\u7ed3\u5408\u4f7f\u7528\u53ef\u4ee5\u589e\u5f3a\u7ec6\u80de\u805a\u7c7b<\/mark>\u548c\u751f\u7269\u6807\u5fd7\u7269\uff08\u57fa\u56e0\u548c\u8f6c\u5ea7\u5b50\uff09\u7684\u53d1\u73b0\uff0c\u4ece\u800c\u66f4\u5168\u9762\u5730\u7406\u89e3\u7ec6\u80de\u7279\u6027\u3002e<\/strong> MATES \u5b9e\u73b0\u57fa\u56e0\u7ec4\u8303\u56f4\u7684\u8bfb\u6bb5\u8986\u76d6\u53ef\u89c6\u5316\uff0c\u5e76\u751f\u6210\u57fa\u4e8e\u57fa\u56e0\u7ec4\u6d4f\u89c8\u5668\u7684\u53ef\u89c6\u5316\u6587\u4ef6\u3002\u8be5\u65b9\u6cd5\u5728\u5355\u4e2a\u7ec6\u80de\u4e2d\u5bf9\u7279\u5b9a\u4f4d\u70b9\u7684 \u8f6c\u5ea7\u5b50\u8fdb\u884c\u5b9a\u91cf\uff0c\u751f\u6210\u5305\u542b\u4ece\u6982\u7387\u5206\u914d\u7684\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u8ba1\u7b97\u7684\u8986\u76d6\u7684 bigwig \u6587\u4ef6\u3002\u8fd9\u4e9b\u6587\u4ef6\u5c06\u72ec\u7279\u548c\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u7684\u8986\u76d6\u4fe1\u606f\u5408\u5e76\uff0c\u751f\u6210\u5168\u9762\u7684 bigwig \u6587\u4ef6\uff0c\u53ef\u901a\u8fc7\u4ea4\u4e92\u5f0f\u57fa\u56e0\u7ec4\u67e5\u770b\u5668\uff08IGV\uff09\u7b49\u5de5\u5177\u5b9e\u73b0\u57fa\u56e0\u7ec4\u8303\u56f4\u7684\u8f6c\u5ea7\u5b50\u8bfb\u6bb5\u53ef\u89c6\u5316\u3002<\/p>\n

\u7ed3\u679c\u5c55\u793a<\/strong><\/p>\n

\u5728\u6211\u4eec\u5bf9 MATES \u7684\u7cfb\u7edf\u6027\u8bc4\u4f30\u4e2d\uff0c\u4f7f\u7528\u4e86\u4e0d\u540c\u6d4b\u5e8f\u5e73\u53f0\u3001\u6a21\u5f0f\u548c\u7269\u79cd\u7684\u591a\u79cd\u5355\u7ec6\u80de\u6570\u636e\u96c6\uff0c\u7ed3\u679c\u8868\u660e MATES \u59cb\u7ec8\u80fd\u591f\u63d0\u4f9b\u66f4\u51c6\u786e\u7684\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u7ed3\u679c\u3002\u9664\u4e86\u66f4\u9ad8\u7684\u7cbe\u786e\u6027\u4e4b\u5916\uff0cMATES \u8fd8\u63d0\u4f9b\u4e86\u7279\u5b9a\u4f4d\u70b9\u6c34\u5e73\u7684\u8f6c\u5ea7\u5b50\u5b9a\u91cf\uff0c\u5e76\u4e14\u80fd\u591f\u5728\u4e0d\u540c\u6d4b\u5e8f\u5e73\u53f0\u548c\u6570\u636e\u6a21\u5f0f\u4e0b\u63a8\u5e7f\u4f7f\u7528\uff0c\u4ece\u800c\u66f4\u5168\u9762\u5730\u7406\u89e3\u8f6c\u5ea7\u5b50\u5728\u7ec6\u80de\u52a8\u6001\u548c\u57fa\u56e0\u8c03\u63a7\u4e2d\u7684\u4f5c\u7528\u3002\u6211\u4eec\u8fd8\u901a\u8fc7 Nanopore \u548c PacBio \u957f\u8bfb\u6bb5\u6d4b\u5e8f\u4ee5\u53ca\u6a21\u62df\u6570\u636e\u5bf9\u65b9\u6cd5\u7684\u9884\u6d4b\u7ed3\u679c\u8fdb\u884c\u4e86\u9a8c\u8bc1\u3002\u901a\u8fc7\u5c06 MATES \u7684\u5355\u7ec6\u80de\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u4e0e\u6a21\u62df\u7684\u771f\u5b9e\u503c\u6216\u957f\u8bfb\u6bb5\u6d4b\u5e8f\u7684\u4ee3\u7406\u771f\u5b9e\u503c\u8fdb\u884c\u6bd4\u8f83\uff0c\u6211\u4eec\u8bc1\u660e\u4e86 MATES \u7684\u51c6\u786e\u6027\u53ca\u5176\u76f8\u8f83\u4e8e\u73b0\u6709\u65b9\u6cd5\u7684\u4f18\u52bf\u3002\u7ed3\u679c\u8868\u660e\uff0cMATES \u5728\u63a2\u7d22\u8f6c\u5ea7\u5b50\u5728\u5355\u7ec6\u80de\u751f\u7269\u5b66\u4e2d\u7684\u4f5c\u7528\u65b9\u9762\u8868\u73b0\u51fa\u8272\uff0c\u5e76\u4e3a\u4e0d\u540c\u5b9e\u9a8c\u80cc\u666f\u4e0b\u7684\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u63d0\u4f9b\u4e86\u4e00\u4e2a\u5b9e\u7528\u7684\u89e3\u51b3\u65b9\u6848\u3002<\/p>\n

<\/a><\/p>\n

\u56fe2: MATES \u589e\u5f3a\u5c0f\u9f20\u5316\u5b66\u91cd\u7f16\u7a0b\uff0810X scRNA-seq\u6570\u636e\uff09\u4e2d\u7684\u7ec6\u80de\u805a\u7c7b<\/mark>\u4e0e\u751f\u7269\u6807\u5fd7\u7269\u53d1\u73b0\u3002<\/p>\n

a, b<\/strong> UMAP \u56fe\u663e\u793a\u901a\u8fc7\u6574\u5408\u8f6c\u5ea7\u5b50\u548c\u57fa\u56e0\uff0cMATES \u5728\u7ec6\u80de\u805a\u7c7b<\/mark>\u4e2d\u7684\u6548\u679c\u3002a \u56fe\u6309 Leiden \u805a\u7c7b<\/mark>\u7ed3\u679c\u7740\u8272\uff0c\u800c b \u56fe\u6309\u91cd\u7f16\u7a0b\u9636\u6bb5\u7740\u8272\uff0c\u7a81\u51fa\u663e\u793a\u4e86\u9274\u5b9a\u7684\u57fa\u56e0\uff08\u7d2b\u8272\uff09\u548c \u8f6c\u5ea7\u5b50\uff08\u7ea2\u8272\uff09\u751f\u7269\u6807\u5fd7\u7269\u3002c, d<\/strong> \u989d\u5916\u7684 UMAP \u56fe\u5f3a\u8c03\u4ec5\u4f7f\u7528\u8f6c\u5ea7\u5b50\u8fdb\u884c\u805a\u7c7b<\/mark>\u7684 MATES \u80fd\u529b\uff0cc \u56fe\u6309 Leiden \u805a\u7c7b<\/mark>\u7740\u8272\uff0cd \u56fe\u6309\u91cd\u7f16\u7a0b\u9636\u6bb5\u7740\u8272\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0cMT2_Mm \u548c MERVL-int \u8f6c\u5ea7\u5b50\u662f Zscan4c\/Zscan4d \u9633\u6027\u7ec6\u80de\u4e2d\u7684\u91cd\u8981\u6807\u5fd7\u7269\uff0c\u4e0e\u5df2\u77e5\u7684 2CLCs \u6807\u5fd7\u4e00\u81f4\u3002e\u00a0<\/strong>\u70b9\u56fe\u5c55\u793a MATES \u9274\u5b9a\u7684\u9636\u6bb5\u7279\u5f02\u6027\u6807\u5fd7\u57fa\u56e0\uff08\u7d2b\u8272\uff09\u548c\u8f6c\u5ea7\u5b50\u9ed1\u8272\uff09\u3002f<\/strong> \u793a\u610f\u56fe\u5c55\u793a MATES \u7684\u6982\u7387\u6027\u65b9\u6cd5\u5982\u4f55\u5c06\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\u5206\u914d\u5230\u7279\u5b9a\u8f6c\u5ea7\u5b50\u4f4d\u70b9\uff0c\u7279\u522b\u662f\u5728 2CLCs \u4e2d\u4e0e Zscan4c\/Zscan4d \u4f4d\u70b9\u76f8\u5173\u7684 MT2_Mm \u548c MERVL-int\u3002g<\/strong> \u6761\u5f62\u56fe\u663e\u793a MT2_Mm \u548c MERVL-int \u5728 Zscan4c\/Zscan4d \u4f4d\u70b9\u7684\u8bfb\u6bb5\u5bcc\u96c6\u60c5\u51b5\u3002\u5bcc\u96c6\u7684 p \u503c\u901a\u8fc7\u5355\u4fa7\u4e8c\u9879\u68c0\u9a8c\u8ba1\u7b97\u3002h\u00a0<\/strong>\u7bb1\u7ebf\u56fe\u6bd4\u8f83 MATES \u5728\u7279\u5b9a\u4f4d\u70b9\u6c34\u5e73\u548c\u4e9a\u5bb6\u65cf\u6c34\u5e73\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u4e0b\u7684\u7ec6\u80de\u805a\u7c7b<\/mark>\u6548\u7387\uff0c\u4f7f\u7528\u8c03\u6574\u5170\u5fb7\u6307\u6570\uff08ARI\uff09\u8bc4\u4f30\u3002<\/p>\n

<\/a><\/p>\n

\u56fe 3\uff1aMATES \u5728 Smart-Seq2 \u5355\u7ec6\u80de RNA-seq \u6570\u636e\u4e2d\u5b9a\u91cf\u4e0e\u75be\u75c5\u76f8\u5173\u7684\u8f6c\u5ea7\u5b50\u8868\u8fbe\u3002<\/p>\n

a, b<\/strong> UMAP \u56fe\u5c55\u793a\u4e86\u57fa\u4e8e\u57fa\u56e0\u548c\u8f6c\u5ea7\u5b50\u6807\u5fd7\u7269\u7684\u7ec6\u80de\u805a\u7c7b<\/mark>\u3002MATES \u6216 Gene+TE \u8868\u793a\u901a\u8fc7 MATES \u5b9a\u91cf\u7684\u57fa\u56e0\u8868\u8fbe\u4e0e\u8f6c\u5ea7\u5b50\u6570\u636e\u76f8\u7ed3\u5408\u3002UMAP \u521d\u59cb\u6309 Leiden \u805a\u7c7b<\/mark>\u7740\u8272\uff08a\uff09\uff0c\u968f\u540e\u6309\u7ec6\u80de\u7c7b\u578b\u53ca\u7279\u5f02\u6807\u5fd7\u7269\u7740\u8272\uff0c\u5305\u62ec\u80bf\u7624\u6807\u5fd7\uff08EGFR\u3001HUERS-P1-int \u548c HERVK-int\uff09\u53ca\u514d\u75ab\u7ec6\u80de\u6807\u5fd7\uff08CD74\u3001LTR2B \u548c LTR40A1\uff09\uff08b\uff09\u3002c, d<\/strong> \u57fa\u4e8e MATES \u5b9a\u91cf\u7684\u8f6c\u5ea7\u5b50\u8868\u8fbe\u751f\u6210\u7684 UMAP \u56fe\uff0c\u5206\u522b\u6309 Leiden \u805a\u7c7b<\/mark>\uff08c\uff09\u548c\u6309\u6807\u5fd7\u7269\uff08\u5982 HERVK-int\uff09\u7740\u8272\u7684\u7ec6\u80de\u7c7b\u578b\uff08d\uff09\u3002e \u70b9\u56fe\u63ed\u793a\u4e86 MATES \u9274\u5b9a\u7684\u6807\u5fd7\u57fa\u56e0\u3001\u8f6c\u5ea7\u5b50\u548c\u7ec6\u80de\u7c7b\u578b\u4e4b\u95f4\u7684\u5173\u8054\u3002f, g<\/strong> \u5c55\u793a\u4e86\u5229\u7528 MATES \u7684\u7279\u5b9a\u4f4d\u70b9\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u589e\u5f3a\u805a\u7c7b<\/mark>\u7cbe\u786e\u6027\u7684\u7ed3\u679c\uff0cf \u56fe\u663e\u793a Leiden \u805a\u7c7b<\/mark>\uff0cg \u56fe\u663e\u793a\u7ec6\u80de\u7c7b\u578b\u3002h<\/strong> \u5217\u51fa\u514d\u75ab\u7ec6\u80de\u4e2d\u7279\u5b9a\u4f4d\u70b9\u6c34\u5e73\u9ad8\u8868\u8fbe\u7684\u8f6c\u5ea7\u5b50\u6807\u5fd7\u7269\uff08LTR2B\uff09\u53ca\u5176\u975e\u8868\u8fbe\u4f4d\u70b9\uff0c\u8bc1\u660e\u4e86 MATES \u5728\u4f4d\u70b9\u6c34\u5e73\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u65b9\u9762\u7684\u80fd\u529b\u3002i<\/strong> \u6761\u5f62\u56fe\u5c55\u793a\u514d\u75ab\u7ec6\u80de\u548c\u80bf\u7624\u7ec6\u80de\u4e2d\u8f6c\u5ea7\u5b50\u7684\u5e73\u5747\u7279\u5b9a\u4f4d\u70b9\u8868\u8fbe\u6c34\u5e73\u3002j<\/strong> \u7bb1\u7ebf\u56fe\u6bd4\u8f83\u4e86\u57fa\u4e8e MATES \u7684\u7279\u5b9a\u4f4d\u70b9\u6c34\u5e73\u548c\u4e9a\u5bb6\u65cf\u6c34\u5e73\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u7684\u7ec6\u80de\u805a\u7c7b<\/mark>\u6548\u679c\uff0c\u901a\u8fc7\u8c03\u6574\u5170\u5fb7\u6307\u6570\uff08ARI\uff09\u8bc4\u4f30\uff0c\u8868\u660e MATES \u5728\u751f\u7269\u6807\u5fd7\u7269\u8bc6\u522b\u548c\u7ec6\u80de\u5206\u7c7b\u4e2d\u7684\u5206\u8fa8\u7387\u63d0\u5347\u3002<\/p>\n

<\/a><\/p>\n

\u56fe 4\uff1aMATES \u5728\u6210\u5e74\u5c0f\u9f20\u5927\u8111 scATAC-seq \u6570\u636e\u4e2d\u7684\u591a\u6837\u6027\u5e94\u7528\u3002<\/p>\n

a\u2013d<\/strong> UMAP \u56fe\u5c55\u793a\u4e86 MATES \u5728\u7ec6\u80de\u805a\u7c7b<\/mark>\u548c\u8bc6\u522b\u7279\u5f81\u6027\u8f6c\u5ea7\u5b50\u6807\u5fd7\u7269\u4e2d\u7684\u5b9a\u91cf\u6548\u679c\uff0c\u7ed3\u5408\u8f6c\u5ea7\u5b50\u548c\u5cf0\u503c\u6570\u636e\u8fdb\u884c\u805a\u7c7b<\/mark>\u5206\u6790\u3002a \u56fe\u663e\u793a Leiden \u805a\u7c7b<\/mark>\u7ed3\u679c\uff0cb \u56fe\u6309\u7ec6\u80de\u7c7b\u578b\u53ca\u8f6c\u5ea7\u5b50\u6807\u5fd7\u7269\u7740\u8272\uff0c\u9274\u5b9a\u51fa\u4e86\u5173\u952e\u8f6c\u5ea7\u5b50\u6807\u5fd7\u7269\uff0c\u4f8b\u5982\u5728\u5de8\u566c\u7ec6\u80de\u4e2d\u7684 RMER16_Mm \u548c RLTR44B\uff0c\u5728\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u4e2d\u7684 MamRep434 \u548c MER124\uff0c\u4ee5\u53ca\u5728\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u4e2d\u7684 MURVY-LTR \u548c MamRep1527\u3002c<\/strong> \u548c d<\/strong> \u56fe\u5c55\u793a\u4e86 MATES \u5728\u4ee5\u8f6c\u5ea7\u5b50\u4e3a\u4e2d\u5fc3\u7684\u805a\u7c7b<\/mark>\u4e2d\u7684\u7279\u5f02\u6027\uff0c\u4ec5\u4f7f\u7528 MATES \u7684\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u6570\u636e\u8fdb\u884c\u805a\u7c7b<\/mark>\u5206\u6790\uff0c\u5176\u4e2d c \u56fe\u805a\u7126\u4e8e Leiden \u805a\u7c7b<\/mark>\uff0cd \u56fe\u5c55\u793a\u7ec6\u80de\u7c7b\u578b\u53ca\u4e4b\u524d\u63d0\u5230\u7684\u7279\u5f81\u6027\u8f6c\u5ea7\u5b50\u6807\u5fd7\u7269\u3002e<\/strong> \u70b9\u56fe\u7b80\u660e\u5448\u73b0\u4e86 MATES \u9274\u5b9a\u7684\u7ec6\u80de\u7c7b\u578b\u7279\u5f02\u6027\u8f6c\u5ea7\u5b50\u6807\u5fd7\u7269\u3002f\u2013h<\/strong> \u8fd9\u4e9b\u9762\u677f\u8bf4\u660e\u4e86 MATES \u4f7f\u7528\u7279\u5b9a\u4f4d\u70b9\u6c34\u5e73\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u63d0\u5347\u805a\u7c7b<\/mark>\u51c6\u786e\u6027\u7684\u6548\u679c\u3002f \u56fe\u5c55\u793a\u4e86\u57fa\u4e8e\u7279\u5b9a\u4f4d\u70b9\u6c34\u5e73\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u7684 UMAP \u53ef\u89c6\u5316\uff0c\u6309\u989c\u8272\u6807\u8bc6 Leiden \u805a\u7c7b<\/mark>\uff1bg \u56fe\u5c55\u793a\u4e86\u76f8\u540c\u7684 UMAP\uff0c\u4f46\u6309\u989c\u8272\u533a\u5206\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\uff1bh \u56fe\u63d0\u4f9b\u4e86\u7279\u5b9a\u4f4d\u70b9\u6c34\u5e73\u7684\u8f6c\u5ea7\u5b50\u6807\u5fd7\u7269 RLTR44B \u5728\u5de8\u566c\u7ec6\u80de\u4e2d\u7684\u5177\u4f53\u793a\u4f8b\uff0c\u5e76\u4e0e\u8be5\u8f6c\u5ea7\u5b50\u4e00\u4e2a\u672a\u5f00\u653e\u4f4d\u70b9\u8fdb\u884c\u5bf9\u6bd4\uff0c\u4f53\u73b0\u4e86 MATES \u5728\u8be6\u7ec6\u7279\u5b9a\u4f4d\u70b9\u6c34\u5e73\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u65b9\u9762\u7684\u80fd\u529b\u3002i<\/strong> \u7bb1\u7ebf\u56fe\u6bd4\u8f83\u4e86 MATES \u5728\u7279\u5b9a\u4f4d\u70b9\u6c34\u5e73\u4e0e\u4e9a\u5bb6\u65cf\u6c34\u5e73\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u4e0b\u7684\u7ec6\u80de\u805a\u7c7b<\/mark>\u6548\u7387\uff08\u8c03\u6574\u5170\u5fb7\u6307\u6570\uff0cARI\uff09\uff0c\u7a81\u51fa\u4e86\u91c7\u7528\u7279\u5b9a\u4f4d\u70b9\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u7684\u4f18\u52bf\u3002j<\/strong> \u6761\u5f62\u56fe\u5c55\u793a\u5de8\u566c\u7ec6\u80de\u3001\u5c11\u7a81\u80f6\u8d28\u7ec6\u80de\u548c\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u4e2d\u7279\u5b9a\u4f4d\u70b9\u8f6c\u5ea7\u5b50\u7684\u5e73\u5747\u8868\u8fbe\u6c34\u5e73\u3002k<\/strong> \u70b9\u56fe\u663e\u793a\u4e86 MATES \u9274\u5b9a\u7684\u7279\u5b9a\u4f4d\u70b9\u6c34\u5e73\u8f6c\u5ea7\u5b50\u6807\u5fd7\u7269\u53ca\u5176\u5bf9\u5e94\u7684\u7ec6\u80de\u7c7b\u578b\u3002<\/p>\n

<\/a><\/p>\n

\u56fe 5\uff1a\u4f7f\u7528 MATES \u5bf9\u4eba\u7c7b PBMCs \u8fdb\u884c\u591a\u6a21\u6001\u8f6c\u5ea7\u5b50\u5206\u6790\u3002<\/p>\n

a, b<\/strong> UMAP \u56fe\u5c55\u793a\u4e86\u901a\u8fc7\u6574\u5408\u00a0scRNA\u00a0\u548c scATAC \u6a21\u6001\u7684 MATES \u805a\u7c7b<\/mark>\u7ed3\u679c\uff0ca \u56fe\u663e\u793a Leiden \u805a\u7c7b<\/mark>\uff0cb \u56fe\u5c55\u793a\u7ec6\u80de\u7c7b\u578b\u805a\u7c7b<\/mark>\u3002c\u2013f<\/strong> \u8de8\u6a21\u6001\u7684\u8f6c\u5ea7\u5b50\u5b9a\u91cf\u7a81\u51fa\u591a\u6a21\u6001\u5b9a\u91cf\u7684\u4e92\u8865\u6027\u3002c \u548c d \u56fe\u5c55\u793a\u4e86\u5728 scRNA \u6a21\u6001\u4e2d\u57fa\u56e0\u548c\u8f6c\u5ea7\u5b50\u8054\u5408\u7684 UMAP \u805a\u7c7b<\/mark>\uff0ce \u548c f \u56fe\u5219\u5c55\u793a\u4e86\u5728 scATAC \u6a21\u6001\u4e2d\u5cf0\u503c\u548c\u8f6c\u5ea7\u5b50\u8054\u5408\u7684 UMAP \u805a\u7c7b<\/mark>\u3002c \u548c e \u6309 Leiden \u805a\u7c7b<\/mark>\u7740\u8272\uff0cd \u548c f \u6309\u7ec6\u80de\u7c7b\u578b\u7740\u8272\uff0c\u5c55\u793a\u4e86\u8f6c\u5ea7\u5b50\u5982 AluYa5 \u5728\u4e24\u79cd\u6a21\u6001\u4e2d\u7684\u5dee\u5f02\u6027\u8868\u8fbe\uff0c\u800c MER48\u3001LTR71A \u548c MER54A \u5219\u7279\u5f02\u4e8e scATAC \u6a21\u6001\u3002g\u2013l<\/strong> \u8fd9\u4e00\u7cfb\u5217 UMAP \u56fe\u548c\u7bb1\u7ebf\u56fe\u8bf4\u660e\u4e86\u591a\u6a21\u6001\u8f6c\u5ea7\u5b50\u5206\u6790\u3002g \u548c j \u662f\u8f6c\u5ea7\u5b50\u8868\u8fbe\u7684 UMAP \u56fe\uff0c\u6309 Leiden \u805a\u7c7b<\/mark>\u7740\u8272\u4ee5\u7a81\u51fa\u805a\u7c7b<\/mark>\u6a21\u5f0f\uff1bh \u548c k \u662f\u805a\u7126\u4e8e\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u53ca\u5176\u8f6c\u5ea7\u5b50\u6807\u5fd7\u7269\u7684 UMAP \u56fe\uff0c\u63d0\u4f9b\u4e86\u7ec6\u80de\u7279\u6027\u548c\u76f8\u5173\u8f6c\u5ea7\u5b50\u7684\u6df1\u5165\u89c1\u89e3\uff1bi \u548c l \u662f\u6bd4\u8f83\u7ec6\u80de\u805a\u7c7b<\/mark>\u6548\u679c\u7684\u7bb1\u7ebf\u56fe\uff08\u8c03\u6574\u5170\u5fb7\u6307\u6570\uff0cARI\uff09\uff0c\u5f3a\u8c03\u7279\u5b9a\u4f4d\u70b9\u6c34\u5e73\u5b9a\u91cf\u76f8\u6bd4\u4e9a\u5bb6\u65cf\u6c34\u5e73\u5b9a\u91cf\u63d0\u4f9b\u7684\u66f4\u9ad8\u5206\u8fa8\u7387\u3002m<\/strong> \u5c55\u793a\u4e86\u901a\u8fc7 scRNA \u548c scATAC \u6a21\u6001\u9274\u5b9a\u7684\u8f6c\u5ea7\u5b50\u6807\u5fd7\u7269\uff0c\u6307\u51fa\u9ad8\u8868\u8fbe\u7684\u8f6c\u5ea7\u5b50\u901a\u5e38\u4e0e\u67d3\u8272\u8d28\u53ef\u53ca\u6027\u589e\u5f3a\u76f8\u5173\uff0c\u800c\u53cd\u5411\u60c5\u51b5\u5e76\u975e\u666e\u904d\u89c2\u5bdf\u5230\uff0c\u7a81\u663e\u4e86\u6bcf\u79cd\u6a21\u6001\u7684\u72ec\u7279\u8d21\u732e\u3002n<\/strong> \u70b9\u56fe\u6355\u6349\u4e86\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u7684\u7279\u5f81\u6027\u8f6c\u5ea7\u5b50\uff0c\u9a8c\u8bc1\u4e86 scATAC \u548c scRNA \u6570\u636e\u7684\u4e92\u8865\u6027\uff0c\u4e3a\u5168\u9762\u4e86\u89e3\u8f6c\u5ea7\u5b50\u52a8\u6001\u63d0\u4f9b\u4e86\u652f\u6301\u3002<\/p>\n

\u603b\u7ed3\u4e0e\u5c55\u671b<\/strong><\/p>\n

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\u8bba\u6587\u94fe\u63a5\uff1ahttps:\/\/www.nature.com\/articles\/s41467-024-53114-7<\/em><\/p>\n

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