{"id":40008,"date":"2025-04-10T00:03:04","date_gmt":"2025-04-09T16:03:04","guid":{"rendered":"https:\/\/17aitech.com\/?p=40008"},"modified":"2025-04-10T00:03:04","modified_gmt":"2025-04-09T16:03:04","slug":"%e9%87%8f%e5%8c%96%e5%8d%95%e4%b8%aa%e7%bb%86%e8%83%9e%e4%b8%ad%e8%a1%a8%e8%be%be%e7%9a%84%e4%b8%80%e5%8d%8a%e8%9b%8b%e7%99%bd%e8%b4%a8%e7%bb%84%ef%bc%8c%e8%b4%a8%e8%b0%b1%e6%8a%80%e6%9c%af%e6%9c%80","status":"publish","type":"post","link":"https:\/\/17aitech.com\/?p=40008","title":{"rendered":"\u91cf\u5316\u5355\u4e2a\u7ec6\u80de\u4e2d\u8868\u8fbe\u7684\u4e00\u534a\u86cb\u767d\u8d28\u7ec4\uff0c\u8d28\u8c31\u6280\u672f\u6700\u65b0\u8fdb\u5c55\u63a8\u52a8SCP\u53d1\u5c55"},"content":{"rendered":"

\u6587\u7ae0\u6765\u6e90\u4e8e\u4e92\u8054\u7f51:\u91cf\u5316\u5355\u4e2a\u7ec6\u80de\u4e2d\u8868\u8fbe\u7684\u4e00\u534a\u86cb\u767d\u8d28\u7ec4\uff0c\u8d28\u8c31\u6280\u672f\u6700\u65b0\u8fdb\u5c55\u63a8\u52a8SCP\u53d1\u5c55<\/a><\/p>\n

<\/a><\/section>\n

\u7f16\u8f91\u4e28coisini<\/span><\/p>\n

\u5355\u7ec6\u80de\u86cb\u767d\u8d28\u7ec4\u5b66\uff08single-cell proteomics\uff0cSCP\uff09\u662f\u7814\u7a76\u5355\u4e2a\u7ec6\u80de\u5185\u86cb\u767d\u8d28\u8868\u8fbe\u591a\u6837\u6027\u7684\u7814\u7a76\u9886\u57df\u3002\u81ea 2020 \u5e74\u4ee5\u6765\uff0c\u86cb\u767d\u8d28\u7ec4\u5b66\u5728\u53ef\u8fbe\u5230\u7684\u7075\u654f\u5ea6\u65b9\u9762\u5b9e\u73b0\u4e86\u8d85\u8fc7 100 \u500d\u7684\u63d0\u5347\uff0c\u8fd9\u4e00\u6210\u5c31\u5f97\u76ca\u4e8e\u8d28\u8c31\u4eea\u8bbe\u8ba1\u7684\u4e00\u7cfb\u5217\u6539\u8fdb\u4ee5\u53ca\u7ed3\u5408\u673a\u5668\u5b66\u4e60\u7684\u6570\u636e\u5904\u7406\u7b97\u6cd5\u3002SCP \u6709\u6f5c\u529b\u6269\u5c55\u73b0\u6709\u5206\u6790\u5de5\u5177\u96c6\uff0c\u4f46\u5728\u541e\u5410\u91cf\u548c\u86cb\u767d\u8d28\u7ec4\u6df1\u5ea6\u65b9\u9762\u4ecd\u53d7\u5230\u9650\u5236\u3002<\/p>\n

\u8fd1\u671f\u4e00\u7bc7\u53d1\u8868\u5728\u300aNature\u300b\u4e0a\uff0c\u9898\u4e3a\u300aEntering the era of deep single-cell proteomics\u300b\u7684\u6587\u7ae0\u4ecb\u7ecd\u4e86\u4e24\u9879\u65b0\u7814\u7a76\uff1aBubis \u7b49\u4eba\u548c Ye \u7b49\u4eba\u5229\u7528\u8d28\u8c31\u6280\u672f\u7684\u6700\u65b0\u8fdb\u5c55\uff0c\u91cf\u5316\u4e86\u5355\u4e2a HeLa \u7ec6\u80de\u4e2d\u8868\u8fbe\u7684\u4e00\u534a\u86cb\u767d\u8d28\u7ec4\uff0c\u541e\u5410\u91cf\u8fbe\u5230 50-120 \u4e2a\u7ec6\u80de \/ \u5929\u3002\u8fd9\u4e00\u8fdb\u5c55\u4ee3\u8868\u7740 SCP \u5728\u7cbe\u786e\u7ed8\u5236\u86cb\u767d\u8d28\u7ec4\u8c03\u63a7\u56fe\u8c31\u65b9\u9762\uff0c\u80fd\u529b\u6709\u4e86\u91cd\u5927\u98de\u8dc3\u3002<\/p>\n

<\/a><\/section>\n

\u6587\u7ae0\u94fe\u63a5\uff1ahttps:\/\/www.nature.com\/articles\/s41592-025-02620-7<\/p>\n

Bubis \u7b49\u4eba\u5229\u7528\u5176\u5e73\u53f0\u8868\u5f81\u4e86\u591a\u80fd\u5e72\u7ec6\u80de\u5728\u5206\u5316\u524d\u540e\u7684\u86cb\u767d\u8d28\u7ec4\uff0c\u6a21\u62df\u4e86\u4e0d\u540c\u7684\u56ca\u80da\u7ec6\u80de\u8c31\u7cfb\uff0c\u5e76\u8fdb\u4e00\u6b65\u5f3a\u8c03\u4e86\u6df1\u5ea6\u5355\u7ec6\u80de\u86cb\u767d\u8d28\u7ec4\u5b66\uff08SCP\uff09\u5728\u9610\u660e\u7ec6\u80de\u5f02\u8d28\u6027\u65b9\u9762\u7684\u80fd\u529b\u3002<\/p>\n

<\/a><\/section>\n

Bubis \u7b49\u4eba\u7814\u7a76\u8bba\u6587\uff1ahttps:\/\/www.nature.com\/articles\/s41592-024-02559-1<\/p>\n

Ye \u7b49\u4eba\u901a\u8fc7\u8ffd\u8e2a\u836f\u7406\u5b66\u523a\u6fc0\u4e0b\u764c\u7ec6\u80de\u7403\u4f53\u7684\u8f6c\u53d8\uff0c\u5c55\u793a\u4e86\u5176\u65b9\u6cd5\u5728\u86cb\u767d\u8d28\u7ec4\u836f\u7269\u9776\u70b9\u89e3\u6790\u4e2d\u7684\u9002\u7528\u6027\u3002\u7814\u7a76\u56e2\u961f\u8fd8\u76d1\u6d4b\u4e86\u4eba\u7c7b\u8bf1\u5bfc\u591a\u80fd\u5e72\u7ec6\u80de\u5411\u80da\u4f53\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u7684\u5206\u5316\u8fc7\u7a0b\u3002<\/p>\n

<\/a><\/section>\n

Ye \u7b49\u4eba\u7814\u7a76\u8bba\u6587\uff1ahttps:\/\/www.nature.com\/articles\/s41592-024-02558-2<\/p>\n

\u4e24\u9879\u7814\u7a76\u90fd\u5229\u7528\u4e86\u6700\u8fd1\u63a8\u51fa\u7684\u00a0Orbitrap Astral\u00a0\u8d28\u8c31\u4eea\uff0c\u8be5\u4eea\u5668\u7ed3\u5408\u4e86\u7528\u4e8e\u672a\u7247\u6bb5\u5316\u80bd\u6bb5\u9ad8\u5206\u8fa8\u7387\u5206\u6790\u7684\u4f20\u7edf Orbitrap \u548c\u65b0\u7684 Astral \u5206\u6790\u5668\uff0c\u540e\u8005\u5728\u9ad8\u8fbe 200 Hz \u7684\u4e32\u8054\u8d28\u8c31\u626b\u63cf\u901f\u5ea6\u4e0b\u4e5f\u80fd\u5b9e\u73b0\u9ad8\u7075\u654f\u5ea6\u548c\u9ad8\u5206\u8fa8\u7387\u3002<\/p>\n

\u8d28\u8c31\u4eea\u7684\u7ec8\u6781\u7075\u654f\u5ea6\u5728\u5355\u7ec6\u80de\u86cb\u767d\u8d28\u7ec4\u5b66\uff08SCP\uff09\u4e2d\u8d77\u5230\u5173\u952e\u4f5c\u7528\uff0c\u4f46\u4ecd\u9762\u4e34\u591a\u4e2a\u95ee\u9898\u3002<\/p>\n

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\u5176\u6b21\uff0c\u6db2\u76f8\u8272\u8c31\uff08LC\uff09\u7cfb\u7edf\u5fc5\u987b\u4f18\u5316\uff0c\u4ee5\u5b9e\u73b0\u9ad8\u541e\u5410\u91cf\u4e0b\u7684\u4f4e\u6d41\u901f\u91c7\u96c6\u3002\u4e24\u9879\u7814\u7a76\u5747\u5728\u4f20\u7edf\u67b6\u6784\u7684 LC \u7cfb\u7edf\uff08Thermo Fisher Scientific Vanquish\uff09\u4e0a\u5efa\u7acb\u4e86\u5de5\u4f5c\u6d41\u7a0b\uff0c\u8be5\u7cfb\u7edf\u4ee5\u6bcf\u5929 30-80 \u4e2a\u6837\u54c1\uff08SPD\uff09\u7684\u541e\u5410\u91cf\u8fd0\u884c\uff0c\u901a\u8fc7\u9ad8\u8fbe 1 \u03bcl\/min \u7684\u6d41\u901f\u5b9e\u73b0\u5feb\u901f\u6837\u54c1\u52a0\u8f7d\u3002\u7cfb\u7edf\u80fd\u591f\u5728 50 SPD \u4e0b\u4ece\u4ec5 250 pg \u7684 HeLa \u80f0\u9176\u6d88\u5316\u7269\u4e2d\u6d4b\u91cf 7500 \u79cd\u86cb\u767d\u8d28\uff0c\u5e76\u5728 60 SPD \u4e0b\u4ece\u5355\u4e2a HeLa \u7ec6\u80de\u4e2d\u6d4b\u91cf 6500 \u79cd\u86cb\u767d\u8d28\u3002Ye \u7b49\u4eba\u8fdb\u4e00\u6b65\u6d4b\u8bd5\u4e86 Evosep One LC\uff0c\u8be5\u7cfb\u7edf\u4f7f\u7528\u9884\u5f62\u6210\u7684\u8272\u8c31\u68af\u5ea6\uff0c\u5728 120 SPD \u4e0b\u5355\u4e2a HeLa \u7ec6\u80de\u4e2d\u68c0\u6d4b\u5230\u8d85\u8fc7 4500 \u79cd\u86cb\u767d\u8d28\u3002<\/p>\n

<\/a><\/section>\n

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