{"id":40067,"date":"2025-04-11T21:02:42","date_gmt":"2025-04-11T13:02:42","guid":{"rendered":"https:\/\/17aitech.com\/?p=40067"},"modified":"2025-04-11T21:02:42","modified_gmt":"2025-04-11T13:02:42","slug":"%e8%a5%bf%e6%b9%96%e5%a4%a7%e5%ad%a6%e5%bc%80%e5%8f%91esm-ezy%ef%bc%9a%e4%b8%80%e7%a7%8d%e7%94%a8%e4%ba%8e%e6%8c%96%e6%8e%98%e9%ab%98%e6%80%a7%e8%83%bd%e9%85%b6%e7%9a%84%e6%b7%b1%e5%ba%a6%e5%ad%a6","status":"publish","type":"post","link":"https:\/\/17aitech.com\/?p=40067","title":{"rendered":"\u897f\u6e56\u5927\u5b66\u5f00\u53d1ESM-Ezy\uff1a\u4e00\u79cd\u7528\u4e8e\u6316\u6398\u9ad8\u6027\u80fd\u9176\u7684\u6df1\u5ea6\u5b66\u4e60\u7b56\u7565"},"content":{"rendered":"

\u6587\u7ae0\u6765\u6e90\u4e8e\u4e92\u8054\u7f51:\u897f\u6e56\u5927\u5b66\u5f00\u53d1ESM-Ezy\uff1a\u4e00\u79cd\u7528\u4e8e\u6316\u6398\u9ad8\u6027\u80fd\u9176\u7684\u6df1\u5ea6\u5b66\u4e60\u7b56\u7565<\/a><\/p>\n

<\/a><\/section>\n

\u56fe\u793a\uff1a\u76f8\u5173\u00a0MCO \u7684\u6676\u4f53\u7ed3\u6784\u3002\uff08\u6765\u6e90\uff1a\u8bba\u6587\uff09<\/p>\n

\u7f16\u8f91 | \u767d\u83dc\u53f6<\/p>\n

UniProt\u00a0\u6570\u636e\u5e93<\/mark>\u662f\u751f\u7269\u50ac\u5316\u5242\u53d1\u73b0\u7684\u5b9d\u8d35\u8d44\u6e90\uff0c\u4f46\u9884\u6d4b\u9176\u529f\u80fd\u4ecd\u7136\u5177\u6709\u6311\u6218\u6027\uff0c\u5c24\u5176\u662f\u5bf9\u4e8e\u4f4e\u76f8\u4f3c\u6027\u5e8f\u5217\u3002\u8bc6\u522b\u5177\u6709\u589e\u5f3a\u50ac\u5316\u6027\u80fd\u7684\u4f18\u8d28\u9176\u66f4\u52a0\u56f0\u96be\u3002<\/p>\n

\u4e3a\u4e86\u514b\u670d\u8fd9\u4e9b\u6311\u6218\uff0c\u897f\u6e56\u5927\u5b66\u7684\u7814\u7a76\u56e2\u961f\u5f00\u53d1\u4e86 ESM-Ezy\uff0c\u8fd9\u662f\u4e00\u79cd\u5229\u7528 ESM-1b \u86cb\u767d\u8d28\u8bed\u8a00\u6a21\u578b<\/mark>\u548c\u8bed\u4e49\u7a7a\u95f4\u76f8\u4f3c\u6027\u8ba1\u7b97\u7684\u9176\u6316\u6398\u7b56\u7565\u3002<\/p>\n

\u5229\u7528 ESM-Ezy\uff0c\u7814\u7a76\u4eba\u5458\u9274\u5b9a\u51fa\u5177\u6709\u4f18\u5f02\u50ac\u5316\u6027\u80fd\u7684\u65b0\u578b\u591a\u94dc\u6c27\u5316\u9176 (MCO)\uff0c\u5728\u81f3\u5c11\u4e00\u9879\u6027\u80fd\uff08\u5305\u62ec\u50ac\u5316\u6548\u7387\u3001\u8010\u70ed\u3001\u8010\u6709\u673a\u6eb6\u5242\u6027\u4ee5\u53ca pH \u7a33\u5b9a\u6027\uff09\u65b9\u9762\u4f18\u4e8e\u67e5\u8be2<\/mark>\u9176 (QE) \u7684\u6210\u529f\u7387\u8fbe\u5230 44%\u3002<\/p>\n

\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c51% \u7684 MCO \u5728\u73af\u5883\u4fee\u590d\u5e94\u7528\u4e2d\u8868\u73b0\u51fa\u8272\uff0c\u5176\u4e2d\u4e00\u4e9b\u8868\u73b0\u51fa\u72ec\u7279\u7684\u7ed3\u6784\u57fa\u5e8f\u548c\u72ec\u7279\u7684\u6d3b\u6027\u4e2d\u5fc3\uff0c\u4ece\u800c\u589e\u5f3a\u4e86\u5176\u529f\u80fd\u3002\u9664 MCO \u5916\uff0c40% \u7684 L-\u5929\u51ac\u9170\u80fa\u9176\u6bd4 QE \u5177\u6709\u66f4\u9ad8\u7684\u6bd4\u6d3b\u6027\u548c\u50ac\u5316\u6548\u7387\u3002<\/p>\n

\u56e0\u6b64\uff0cESM-Ezy \u4e3a\u53d1\u73b0\u5177\u6709\u4f4e\u5e8f\u5217\u76f8\u4f3c\u6027\u7684\u9ad8\u6027\u80fd\u751f\u7269\u50ac\u5316\u5242\u63d0\u4f9b\u4e86\u4e00\u79cd\u6709\u524d\u666f\u7684\u65b9\u6cd5\u3002<\/p>\n

\u8be5\u7814\u7a76\u4ee5\u300cESM-Ezy: a deep learning strategy for the mining of novel multicopper oxidases with superior properties<\/em>\u300d\u4e3a\u9898\uff0c\u4e8e 2025 \u5e74 4 \u6708 6 \u65e5\u53d1\u5e03\u5728\u300aNature Communications<\/em>\u300b\u3002<\/p>\n

<\/a><\/section>\n

\u79d1\u5b66\u5bb6\u901a\u5e38\u4f1a\u4ece\u901a\u8fc7\u5e8f\u5217\u76f8\u4f3c\u6027\u7f51\u7edc\uff08SSN\uff09\u5206\u6790\u786e\u5b9a\u7684\u7c07\u4e2d\u968f\u673a\u9009\u62e9\u5019\u9009\u9176\u3002\u7136\u800c\uff0c\u8fd9\u79cd\u53cd\u590d\u8bd5\u9a8c\u7684\u65b9\u6cd5\u901a\u5e38\u6548\u7387\u4f4e\u4e0b\uff0c\u5e76\u4e14\u4f1a\u5ef6\u957f\u53d1\u73b0\u8fc7\u7a0b\u3002<\/p>\n

\u6b64\u5916\uff0c\u5c3d\u7ba1 PLM \u6700\u8fd1\u5df2\u6210\u529f\u7528\u4e8e\u6307\u5bfc\u86cb\u767d\u8d28\u5de5\u7a0b\u3001\u6297\u4f53\u8bbe\u8ba1\u3001\u9176\u529f\u80fd\u8bc4\u4f30\uff08\u5982 CLEAN \u548c TM-Vec\uff09\u548c\u8fdc\u7a0b\u540c\u6e90\u6027\u68c0\u6d4b\uff08\u5982 PLMSearch\uff09\uff0c\u4f46\u5b83\u4eec\u5c1a\u672a\u7528\u4e8e\u63a2\u7d22\u4f4e\u5e8f\u5217\u540c\u6e90\u6027\u533a\u57df\u4e2d\u9176\u7684\u50ac\u5316\u7279\u6027\u3002\u897f\u6e56\u5927\u5b66\u56e2\u961f\u5f00\u53d1\u7684 ESM-Ezy \u89e3\u51b3\u4e86\u8fd9\u4e9b\u4ee5\u524d\u672a\u89e3\u51b3\u7684\u6311\u6218\u3002<\/p>\n

<\/a><\/section>\n

\u56fe\u793a\uff1aESM-Ezy \u5de5\u4f5c\u6d41\u7a0b\u3002\uff08\u6765\u6e90\uff1a\u8bba\u6587\uff09<\/p>\n

ESM-Ezy \u662f\u4e00\u79cd\u7531 ESM-1b \u8f85\u52a9\u7684\u6df1\u5ea6\u5b66\u4e60<\/mark>\u7b56\u7565\uff0c\u7528\u4e8e\u63a2\u7d22\u5177\u6709\u4f4e\u5e8f\u5217\u76f8\u4f3c\u6027\u7684\u65b0\u578b MCO\uff0c\u4ece\u800c\u4ee5\u9ad8\u6210\u529f\u7387\u6539\u5584\u50ac\u5316\u6027\u80fd\u3002<\/p>\n

\u901a\u8fc7\u4f7f\u7528\u5c0f\u578b\u4f46\u9ad8\u8d28\u91cf\u7684\u6570\u636e\u96c6\u5bf9 ESM-1b \u8fdb\u884c\u5fae\u8c03\uff0c\u5e76\u6839\u636e\u4e0e\u67e5\u8be2<\/mark>\u9176\uff08QE\uff09\u7684\u8f83\u77ed\u6b27\u51e0\u91cc\u5f97\u8ddd\u79bb<\/mark>\u9009\u62e9\u5019\u9009\u8005\uff0c\u7814\u7a76\u4eba\u5458\u6210\u529f\u8bc6\u522b\u51fa\u5177\u6709\u4f4e\u5e8f\u5217\u76f8\u4f3c\u5ea6\uff08\u5927\u591a\u4f4e\u4e8e 35%\uff09\u7684\u65b0 MCO\uff0c\u8868\u73b0\u51fa\u4f18\u5f02\u7684\u50ac\u5316\u6027\u80fd\u3002<\/p>\n

<\/a><\/section>\n

\u56fe\u793a\uff1a\u5bf9\u65b0\u6316\u6398\u7684 MCO \u548c QE \u8fdb\u884c\u8bc4\u4f30\u3002\uff08\u6765\u6e90\uff1a\u8bba\u6587\uff09<\/p>\n

\u51e0\u4e4e 89% \u7684\u6d4b\u8bd5 MCO \u6210\u529f\u50ac\u5316\u4e86 2,2′-\u8fde\u6c2e-\u53cc\uff083-\u4e59\u57fa\u82ef\u5e76\u567b\u5511\u5549-6-\u78fa\u9178\uff09\u4e8c\u94f5\u76d0 (ABTS) \u6c27\u5316\uff0c\u5176\u4e2d 44% \u4e0e QE \u76f8\u6bd4\uff0c\u81f3\u5c11\u4e00\u79cd\u50ac\u5316\u6027\u80fd\u6709\u6240\u589e\u5f3a\uff0c\u5305\u62ec\u66f4\u9ad8\u7684\u50ac\u5316\u6548\u7387\u3001\u66f4\u597d\u7684\u70ed\u548c\u6709\u673a\u6eb6\u5242\u8010\u53d7\u6027\u4ee5\u53ca\u66f4\u5bbd\u7684 pH \u8303\u56f4\u3002<\/p>\n

\u7814\u7a76\u4eba\u5458\u53d1\u73b0\u786b\u662f\u8fc4\u4eca\u4e3a\u6b62\u62a5\u9053\u7684\u6700\u8010\u70ed\u7684 MCO \u4e4b\u4e00\uff0c\u5728 80\u00b0C \u4e0b\u534a\u8870\u671f\u957f\u8fbe 156.9 \u5206\u949f\uff0c\u5e76\u9996\u6b21\u7528\u72ec\u7279\u7684 Cu-Mn \u6742\u539f\u5b50\u4e2d\u5fc3\u8868\u5f81\u4e86 Bfre\u3002<\/p>\n

\u8be5\u56e2\u961f\u8fd8\u8bc4\u4f30\u4e86\u65b0\u53d1\u73b0\u7684 MCO \u5728\u65e0\u4ecb\u8d28\u751f\u7269\u4fee\u590d\u5e94\u7528\u4e2d\u7684\u6027\u80fd\u300253% \u7684 MCO \u6bd4 QE \u66f4\u6709\u6548\u5730\u4f7f Remazol Brilliant Blue R (RBBR) \u8131\u8272\u3002Talbi \u5728\u5ba4\u6e29\u4e0b\u964d\u89e3\u4e86\u6c2f\u9709\u7d20 (CAP)\uff0c\u5176\u6027\u80fd\u4f18\u4e8e\u4e4b\u524d\u7684\u771f\u83cc\u7cfb\u7edf\u3002<\/p>\n

\u6b64\u5916\uff0cBcece \u5728\u5b75\u80b2 48 \u5c0f\u65f6\u540e\u663e\u793a\u51fa\u964d\u89e3 39.4% \u9ec4\u66f2\u9709\u6bd2\u7d20 B1 (AFB1) \u7684\u80fd\u529b\uff0c\u4e0e\u5176\u4ed6\u7ec6\u83cc MCO \u5728\u6e29\u548c\u6761\u4ef6\u4e0b\u76f8\u6bd4\uff0c\u8868\u73b0\u51fa\u66f4\u4f18\u5f02\u7684\u65e0\u4ecb\u8d28\u964d\u89e3\u6548\u7387\u3002<\/p>\n

\u4e3a\u4e86\u8bc4\u4f30 ESM-Ezy \u65b9\u6cd5\u7684\u666e\u904d\u9002\u7528\u6027\uff0c\u7814\u7a76\u4eba\u5458\u5c06\u5176\u5e94\u7528\u4e8e l-\u5929\u51ac\u9170\u80fa\u9176\uff08l-\u5929\u51ac\u9170\u80fa\u9170\u80fa\u6c34\u89e3\u9176\uff1bEC 3.5.1.1\uff09\uff0c\u8be5\u9176\u53ef\u50ac\u5316 l-\u5929\u51ac\u9170\u80fa\u6c34\u89e3\u4e3a\u6c28\u548c\u00a0l-\u5929\u51ac\u6c28\u9178\u3002\u8fd9\u79cd\u9176\u5df2\u663e\u793a\u51fa\u663e\u8457\u7684\u6cbb\u7597\u6f5c\u529b\uff0c\u7279\u522b\u662f\u5728\u6cbb\u7597\u513f\u7ae5\u6025\u6027\u6dcb\u5df4\u7ec6\u80de\u767d\u8840\u75c5\u65b9\u9762\u3002<\/p>\n

<\/a><\/section>\n

\u56fe\u793a\uff1a\u5bf9\u65b0\u6316\u6398\u7684 MCO \u8fdb\u884c\u751f\u7269\u4fee\u590d\u7684\u8bc4\u4f30\u3002\uff08\u6765\u6e90\uff1a\u8bba\u6587\uff09<\/p>\n

\u4ed6\u4eec\u5bf9 ESM-1b \u8fdb\u884c\u4e86\u5fae\u8c03\u7528\u4e8e\u641c\u7d22 l-\u5929\u51ac\u9170\u80fa\u9176\u3002\u4ed6\u4eec\u9009\u62e9\u4e86\u6765\u81ea\u67af\u8349\u82bd\u5b62\u6746\u83cc 168 \u7684 l-\u5929\u51ac\u9170\u80fa\u9176 (UniProt: O34482) \u4f5c\u4e3a QE\uff0c\u5e76\u786e\u5b9a\u4e86\u4e94\u79cd\u5019\u9009\u9176\u3002<\/p>\n

\u4e0e QE \u76f8\u6bd4\uff0cA0A3N5F6J4 \u548c H1D2G7 \u5206\u522b\u8868\u73b0\u51fa\u7ea6 2.0 \u500d\u548c 4.1 \u500d\u7684\u6bd4\u6d3b\u6027\u4ee5\u53ca 2.0 \u500d\u548c 3.0 \u500d\u7684 kcat\u3002\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0cESM-Ezy \u53ef\u4ee5\u6210\u529f\u8bc6\u522b\u9664\u6c27\u5316\u8fd8\u539f\u9176\u4e4b\u5916\u5177\u6709\u4f18\u5f02\u50ac\u5316\u6027\u80fd\u7684\u9176\u3002<\/p>\n

\u603b\u4f53\u800c\u8a00\uff0cESM-Ezy \u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u4e30\u5bcc\u4e86 MCO \u5e93\uff0c\u63d0\u9ad8\u4e86\u50ac\u5316\u6d3b\u6027\u5e76\u589e\u52a0\u4e86\u7ed3\u6784\u591a\u6837\u6027\uff0c\u4fc3\u8fdb\u4e86\u5b83\u4eec\u5728\u5404\u79cd\u5de5\u4e1a\u73af\u5883\u4e2d\u7684\u5e94\u7528\u3002<\/p>\n

ESM-Ezy\uff1ahttps:\/\/github.com\/westlake-repl\/ESM-Ezy<\/p>\n

\u8bba\u6587\u94fe\u63a5\uff1ahttps:\/\/www.nature.com\/articles\/s41467-025-58521-y<\/p>\n

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